Search for: All records

Protein-based virus-like particles (P-VLPs) are commonly used to spatially organize antigens and enhance humoral immunity through multivalent antigen display. However, P-VLPs are thymus-dependent antigens that are themselves immunogenic and can induce B cell responses that may neutralize the platform. Here, we investigate thymus-independent DNA origami as an alternative material for multivalent antigen display using the receptor binding domain (RBD) of the SARS-CoV-2 spike protein, the primary target of neutralizing antibody responses. Sequential immunization of mice with DNA-based VLPs (DNA-VLPs) elicits protective neutralizing antibodies to SARS-CoV-2 in a manner that depends on the valency of the antigen displayed and on T cell help. Importantly, the immune sera do not contain boosted, class-switched antibodies against the DNA scaffold, in contrast to P-VLPs that elicit strong B cell memory against both the target antigen and the scaffold. Thus, DNA-VLPs enhance target antigen immunogenicity without generating scaffold-directed immunity and thereby offer an important alternative material for particulate vaccine design.

A striking feature of the Earth system is that the Northern and Southern Hemispheres reflect identical amounts of sunlight. This hemispheric albedo symmetry comprises two asymmetries: The Northern Hemisphere is more reflective in clear skies, whereas the Southern Hemisphere is cloudier. Here we show that the hemispheric reflection contrast from differences in continental coverage is offset by greater reflection from the Antarctic than the Arctic, allowing the net clear-sky asymmetry to be dominated by aerosol. Climate model simulations suggest that historical anthropogenic aerosol emissions drove a large increase in the clear-sky asymmetry that would reverse in future low-emission scenarios. High-emission scenarios also show decreasing asymmetry, instead driven by declines in Northern Hemisphere ice and snow cover. Strong clear-sky hemispheric albedo asymmetry is therefore a transient feature of Earth’s climate. If all-sky symmetry is maintained, compensating cloud changes would have uncertain but important implications for Earth’s energy balance and hydrological cycle.

The influence of aerosol particles on cloud reflectivity remains one of the largest sources of uncertainty in our understanding of anthropogenic climate change. Commercial shipping constitutes a large and concentrated aerosol perturbation in a meteorological regime where clouds have a disproportionally large effect on climate. Yet, to date, studies have been unable to detect climatologically relevant cloud radiative effects from shipping, despite models indicating that the cloud response should produce a sizable negative radiative forcing (perturbation to Earth's energy balance). We attribute a significant increase in cloud reflectivity to enhanced cloud droplet number concentrations within a major shipping corridor in the southeast Atlantic. Prevailing winds constrain emissions around the corridor, which cuts through a climatically important region of expansive low cloud cover. We use universal kriging, a classic geostatistical method, to estimate what cloud properties would have been in the absence of shipping. In the morning, cloud brightening is consistent with changes in microphysics alone, whereas in the afternoon, increases in cloud brightness from microphysical changes are offset by decreases in the total amount of cloud water. We calculate an effective radiative forcing within the southeast Atlantic shipping corridor of approximately −2 W/m². Several years of data are required to identify a clear signal.

Extrapolating our results globally, we calculate an effective radiative forcing due to aerosol‐cloud interactions in low clouds of −1.0 W/m²(95% confidence interval: −1.6 to −0.4 W/m²). The unique setup in the southeast Atlantic could be an ideal test for the representation of aerosol‐cloud interactions in climate models.